Evaluating pollen competition in Douglas-fir using a chloroplast DNA marker

An experiment was conducted to test pollen competition as reflected in the reproductive success of individual pollen lots in two polymixes in Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco). One polymix contained six pollen lots in equal proportion by weight, the other polymix was formulated usin...

Full description

Saved in:
Bibliographic Details
Published inForest Genetics (Zvolen) Vol. 6; no. 1
Main Authors Stoehr, M.U, Mullen, M.C, Harrison, D.L.S, Webber, J.E. (Ministry of Forests, Glyn Road Research Station (Canada))
Format Journal Article
LanguageEnglish
Published 1999
Subjects
AMENAGEMENT FORESTIER
CHLOROPHYLLE
CHLOROPHYLLS
CLOROFILAS
FOREST MANAGEMENT
GENETIC MARKERS
MARCADORES GENETICOS
MARQUEUR GENETIQUE
ORDENACION FORESTAL
PEUPLEMENT SEMENCIER
POLEN
POLINIZACION
POLLEN
POLLINATION
POLLINISATION
PRODUCCION
PRODUCTION
PSEUDOTSUGA MENZIESII
REPRODUCCION
REPRODUCTION
RESPIRACION
RESPIRATION
RODAL SEMILLERO
SEED
SEED STANDS
SEMENCE
SEMILLAS
Online AccessGet more information

Cover

More Information
Summary:An experiment was conducted to test pollen competition as reflected in the reproductive success of individual pollen lots in two polymixes in Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco). One polymix contained six pollen lots in equal proportion by weight, the other polymix was formulated using the same six pollen lots but in inversely proportional amounts to their viability. Pollen lot viability was measured as respiration (oxygen uptake). Reproductive success of individual male parents (pollen lots) was determined following control pollinations using both polymixes separately on six female clones. The paternity analysis was carried out using a chloroplast DNA marker amplified by the polymerase chain reaction (PCR) in total DNA extracts of embryos dissected from mature seed, allowing the identification of the male parent in "polycrossed" seed. Average number of filled seed per cone was not affected by the two polymixes, but the reproductive success of individual males in the polymixes varied greatly. Progeny arrays for both polymixes showed reproductive bias with reproductive success strongly correlated with pollen viability (r2=0.9)
Bibliography:F30
2000000188
ISSN:1335-048X