Bioluminescence assay in determination of total number of microorganisms in raw milk

• Published in: Bulletin Potravinarskeho Vyskumu (Slovak Republic) Vol. 37; no. 4
• Main Authors: Vytrasova, J, Lendakova, M. (Fakulta Chemicko-technologicka, Pardubice (Czech Republic). Katedra Biologickych a Biochemickych Ved)
• Format: Journal Article
• Language: Slovak
• Published: 1998
• Subjects: ATP; CELL COUNTING; CONTEO DE CELULAS; IMMUNOLOGICAL TECHNIQUES; LAIT; LECHE; MICROORGANISME; MICROORGANISMOS; MICROORGANISMS; MILK; NUMERATION CELLULAIRE; TECHNIQUE IMMUNOLOGIQUE; TECNICAS INMUNOLOGICAS

Total number of microorganisms in raw milk was followed by means of the luminescent method by measuring of the light emission, proportional to the concentration of intracellular ATP. Having removed non-microbial ATP, the amount of ATP released from bacterial cells was indicated using the enzyme/co-e...