Bioluminescence assay in determination of total number of microorganisms in raw milk

Total number of microorganisms in raw milk was followed by means of the luminescent method by measuring of the light emission, proportional to the concentration of intracellular ATP. Having removed non-microbial ATP, the amount of ATP released from bacterial cells was indicated using the enzyme/co-e...

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Bibliographic Details
Published inBulletin Potravinarskeho Vyskumu (Slovak Republic) Vol. 37; no. 4
Main Authors Vytrasova, J, Lendakova, M. (Fakulta Chemicko-technologicka, Pardubice (Czech Republic). Katedra Biologickych a Biochemickych Ved)
Format Journal Article
LanguageSlovak
Published 1998
Subjects
ATP
CELL COUNTING
CONTEO DE CELULAS
IMMUNOLOGICAL TECHNIQUES
LAIT
LECHE
MICROORGANISME
MICROORGANISMOS
MICROORGANISMS
MILK
NUMERATION CELLULAIRE
TECHNIQUE IMMUNOLOGIQUE
TECNICAS INMUNOLOGICAS
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Summary:Total number of microorganisms in raw milk was followed by means of the luminescent method by measuring of the light emission, proportional to the concentration of intracellular ATP. Having removed non-microbial ATP, the amount of ATP released from bacterial cells was indicated using the enzyme/co-enzyme complex, luciferase-luciferin, the addition of which initiated the luminescence. The results obtained were compared with those measured simultaneously using both the automatic method (Bactosan) and the standard plating method. For given type of luminometer, the ATP determination was optimized and interferences were eliminated. The differences in results of these three methods were statistically insignificant
Bibliography:U30
Q04
1999000102
ISSN:0231-9950