Eu3+-labeled Staphylococcus aureus protein as reagent for the determination of specific antibodies

In this work the conditions of labeling protein A with europium ions were studied and the conjugates obtained in this study were compared with traditional peroxidase conjugates currently used in immunochemistry. The conjugates of protein A with Eu3+ chelate were obtained with the use of cyclic dianh...

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Published inZhurnal mikrobiologii, ehpidemiologii i immunobiologii (Russian Federation) no. 4
Main Authors Guzaeva, T.V, Komarov, A.M, Yurov, S.V, Pchelintsev, S.Yu, Chudinov, A.V, Afanas'ev, S.S, Zav'yalov, V.P
Format Publication
LanguageRussian
Published 1994
Subjects
ENFERMEDADES DE LOS ANIMALES
ENFERMEDADES HUMANAS
ENFERMEDADES INFECCIOSAS
IMMUNODIAGNOSTIC
INMUNODIAGNOSTICO
MALADIE DE L'HOMME
MALADIE DES ANIMAUX
MALADIE INFECTIEUSE
PROTEINAS UNICELULARES
PROTEINE MICROBIOLOGIQUE
REPONSE IMMUNITAIRE
RESPUESTA INMUNOLOGICA
STAPHYLOCOCCUS AUREUS
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Summary:In this work the conditions of labeling protein A with europium ions were studied and the conjugates obtained in this study were compared with traditional peroxidase conjugates currently used in immunochemistry. The conjugates of protein A with Eu3+ chelate were obtained with the use of cyclic dianhydride of diethylenetriaminepentaacetic acid (DADETRA). Conjugation methods with the use of DADETRA were shown to permit obtaining high-quality conjugates with europium chelates. Europium-labeled protein A ensured the sensitivity of the determination of adsorbed IgG at a level of 2 ng/ml and the dynamic analytical range within 3 - 1,000 ng/ml, which essentially exceeded similar characteristics of peroxidase conjugates with protein A. Europium-labeled protein A was used for the detection of antibodies to Francisella tularensis in the sera of humans immunized against tularemia. The sensitivity of this assay exceeded that of the enzyme immunoassay 10- to 40- fold. A conclusion was made on the possibility of using europium labelled protein A for the determination of specific antibodies to F. tularensis. This preparation may be useful in the determination of specific antibodies in low-immune sera
Bibliography:9503241
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