Comparative genome analysis in pea (Pisum sativum L.) varieties and lines with chromosomal and molecular markers

• Published in: Генетика (Российская Федерация) no. 12; pp. 1644 - 1651
• Main Authors: Borisov, A.Yu, Tihonovich, I.A., All-Russia Research and Development Inst. of Agricultural Microbiology, St.-Petersburg (Russian Federation), Muravenko, O.V
• Format: Journal Article
• Language: Russian
• Published: 2008
• Subjects: CARIOTIPOS; CARYOTYPE; CHROMOSOME; CHROMOSOMES; CROMOSOMAS; FEDERACION DE RUSIA; FEDERATION DE RUSSIE; GENETIC MARKERS; GENOMAS; GENOME; GENOMES; GENOTIPOS; GENOTYPE; GENOTYPES; http://www.fao.org/aos/agrovoc#c_1598; http://www.fao.org/aos/agrovoc#c_24030; http://www.fao.org/aos/agrovoc#c_3224; http://www.fao.org/aos/agrovoc#c_3225; http://www.fao.org/aos/agrovoc#c_32486; http://www.fao.org/aos/agrovoc#c_33240; http://www.fao.org/aos/agrovoc#c_34079; http://www.fao.org/aos/agrovoc#c_36341; http://www.fao.org/aos/agrovoc#c_3791; http://www.fao.org/aos/agrovoc#c_5933; http://www.fao.org/aos/agrovoc#c_6088; http://www.fao.org/aos/agrovoc#c_8157; IDENTIFICACION; IDENTIFICATION; KARYOTYPES; MARCADORES GENETICOS; MARQUEUR GENETIQUE; PCR; PISUM SATIVUM; POLIMORFISMO; POLYMORPHISM; POLYMORPHISME; RAPD; RUSSIAN FEDERATION; VARIEDADES; VARIETE; VARIETIES
• ISSN: 0016-6758

С banding, Ag-NOR staining, FISH with pTa71 (45S rDN A) and pTa794 (5S rDNA) and RAPD-PCR analysis were used to study the genome and chromosome polymorphism in four varieties (Frisson, Sparkle, Rondo, and Finale) and two genetic lines (Sprint-2 and SGE) of pea Pisum sativum L. A comparison of the C-banding patterns did not reveal any polymorphism within the varieties. The most significantive between-variety differences were observed for the size of С bands on satellite chromosomes 4 and 7. All grain pea varieties (Frisson, Sparkle and Rondo) had a large С band in the satellite of chromosome 4 and a medium С band in the region adjacent to the satellite thread on chromosome 7. С bands were almost of the same size in the genetic lines and vegetable variety Finale. In all accessions 45S rDNA mapped to the secondary constriction regions of chromosomes 1, 3, and 5. The signal from chromosome 5 in the lines was more intense than in the varieties. Ag-NOR staining showed that the transcriptional activity of the 45S rRN A genes on chromosome 7 was higher than on chromosome 4 in all accessions. No more than four Ag-NOR-positive nucleoli were observed in interphase nuclei