Polymorphism of B-lacto globulin gene in Barki sheep breed

• Published in: Biotechnology in Animal Husbandry (Serbia) Vol. 28; no. 2
• Main Authors: El-Hanafy, A.A., King Abdulaziz University, Jeddah (Saudi Arabia). Faculty of Science, Department of Biological Sciences, Sabir, J.S.M., King Abdulaziz University, Jeddah (Saudi Arabia). Faculty of Science, Department of Biological Sciences, Mutawakil, M.H.Z., King Abdulaziz University, Jeddah (Saudi Arabia). Faculty of Science, Department of Biological Sciences, Abo Elsoud, M.E., King Abdulaziz University, Jeddah (Saudi Arabia). Faculty of Science, Department of Biological Sciences, Abdel-Sadek, M.A., King Abdulaziz University, Jeddah (Saudi Arabia). Faculty of Science, Department of Biological Sciences
• Format: Journal Article
• Language: English
• Published: 2012
• Subjects: AEGYPTE; BREEDS (ANIMALS); EGIPTO; EGYPT; GANE; GENES; GENETIC POLYMORPHISM; http://www.fao.org/aos/agrovoc#c_1081; http://www.fao.org/aos/agrovoc#c_15998; http://www.fao.org/aos/agrovoc#c_24031; http://www.fao.org/aos/agrovoc#c_2503; http://www.fao.org/aos/agrovoc#c_3214; http://www.fao.org/aos/agrovoc#c_34079; http://www.fao.org/aos/agrovoc#c_34255; http://www.fao.org/aos/agrovoc#c_7030; MILK YIELD; OVIN; OVINOS; PCR; POLIMORFISMO GENATICO; POLYMORPHISME GANATIQUE; RACE (ANIMAL); RAZAS (ANIMALES); RENDEMENT LAITIER; RENDIMIENTO LECHERO; RFLP; SHEEP
• ISSN: 1450-9156

B - LG gene polymorphism at the DNA level has been analyzed by PCR-RFLP and reported two genetic variants of the B -LG gene in Pag native and Leccese sheep breeds. However, no study has been reported in Barki Egyptian local sheep breed regarding B -LG polymorphism at DNA level. The association of B- LG polymorphism with milk production and manufacturing properties of the cheese in sheep have been extensively studied. However, the results are conflicting, indicating either superiority of a given B - LG variant or absence of relationship. The fingerprinting of B -LG gene and this relation has not clarified yet in local Barki and other sheep breeds raised in Egypt. Regarding the importance of Barki sheep breed as a local adaptive breed in Egypt, this preliminary study aimed to fingerprint B-LG gene in the Barki sheep breed, in an attempt to have clear image about genotypes of B-LG gene in this breed. A total of 60 adult females Barki ewes were fingerprinted for B-LG gene by the PCR-RFLP method. The sheep are raised by farmers in North West coast (Borg EL-Arab, Alexandria, Egypt) in which sheep are living in natural habitats. The amplified product was observed as 120 bp and the restriction digestion with Rsa I revealed three genotypes, namely AA, AB and BB at the B-LG locus. The frequencies of AA, AB and BB genotypes were 0.45, 0.30, 0.25, respectively.