Effect of medium pH and cultivation period on mycelial biomass, polysaccharide, and ligninolytic enzyme production by Ganoderma lucidum from Montengero

• Published in: Archives of biological sciences Vol. 58; no. 3
• Main Authors: Vukojevic, J., University of Belgrade, Belgrade (Serbia). Faculty of Biology, Institute of Botany, Stajic, M., University of Belgrade, Belgrade (Serbia). Faculty of Biology, Institute of Botany, Duletic-Lausevic, S., University of Belgrade, Belgrade (Serbia). Faculty of Biology, Institute of Botany, Simonic, J., University of Belgrade, Belgrade (Serbia). Faculty of Biology, Institute of Botany
• Format: Journal Article
• Language: English
• Published: 2006
• Subjects: BIOMASA; BIOMASS; BIOMASSE; BOSQUE DE CONIFERAS; CONIFEROUS FORESTS; ENZIMAS; ENZYME; ENZYMES; FORET RESINEUSE; GANODERMA LUCIDUM; http://www.fao.org/aos/agrovoc#c_1813; http://www.fao.org/aos/agrovoc#c_24753; http://www.fao.org/aos/agrovoc#c_2603; http://www.fao.org/aos/agrovoc#c_37245; http://www.fao.org/aos/agrovoc#c_49907; http://www.fao.org/aos/agrovoc#c_5017; http://www.fao.org/aos/agrovoc#c_5753; http://www.fao.org/aos/agrovoc#c_6097; http://www.fao.org/aos/agrovoc#c_926; LIGNINOLYTIC MICROORGANISMS; MICELIO; MICRO-ORGANISME LIGNINOLYTIQUE; MICROORGANISMOS LIGNINOLITICOS; MONTENEGRO; MYCELIUM; POLISACARIDOS; POLYHOLOSIDE; POLYSACCHARIDES
• ISSN: 0354-4664

The effect of initial medium pH on biomass, extracellular and intracellular polysaccharide, and ligninolytic enzyme production by Ganoderma lucidum was investigated at different pH values after 7 and 14 days of cultivation. Maximum production of biomass was recorded at pH 4.5 and 5.0; maximum production of extracellular polysaccharides at pH 7.0 and 3.0; and maximal production of intracellular polysaccharides at pH 7.0 aand 5.5. Ligninolytic enzymes were not produced at any pH of the medium. Maximal biomass production was obtained on the 11th day of cultivation; maximal extracellular polysaccharide production on the 7th day; and maximum intracellular polysaccharide production on the 6th and 10th day of cultivation.