Insertion of a CACTA transposable element leads to somatic variegation and germinal instability of p1 gene expression in the absence of element excision
Here we report the molecular analysis of a new p1 mutant allele, p1-vv85, that showed both somatic and germinal instability. A novel CACTA transposom-like element was isolated from the second intron of the p1 gene in both the mutant p1-vv85 and its stable pigmented revertant allele. Transcriptional...
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Main Author | |
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Format | Book |
Language | English Serbian |
Published |
Beograd (Serbia)
Drustvo geneticara Srbije
2007
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Subjects | |
Online Access | Get more information |
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Summary: | Here we report the molecular analysis of a new p1 mutant allele, p1-vv85, that showed both somatic and germinal instability. A novel CACTA transposom-like element was isolated from the second intron of the p1 gene in both the mutant p1-vv85 and its stable pigmented revertant allele. Transcriptional analysis indicated that transcription of the p1 gene in p1-vv85 terminated prematurely in the vinicity of the CACTA insertion site. The results are consistent with a model in which binding of a suppressor-like factor to the CACTA insertion element interferes with transcription elongation. We propose that the reversion of p1-vv85 to a functional P1-rr state does not result from transposable element excision, but rather from relief of suppression which is correlated with methylation of the CACTA insertion element. |
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Bibliography: | COBISS.SR-ID 143994380 F30 978-86-87109-00-1 |
ISBN: | 9788687109001 8687109008 |