Restriction fragment length polymorphism study of some descendants of H. rigidus (Cass) Desf. x H. annuus L. interspecific hybrid

Eighteen plants of interspecific hybrid H. rigidus (Cass) Desf. x H. annuus L. have been studied in connection with phenotypical characteristics suggesting the introgression of some traits from wilds into cultivated sunflower. Every genotype has been characterized by phenotypical value and by its RF...

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Published inAnalele Institutului de Cercetari pentru Cereale si Plante Tehnice Fundulea (Romania) Vol. 61
Main Authors Iuoras, M, Vranceanu, A.V. (Institutul de Cercetari pentru Cereale si Plante Tehnice, Fundulea (Romania))
Format Journal Article
LanguageRomanian
Published 1994
Subjects
ADN
ENZIMAS
ENZYME
GENOTIPOS
GENOTYPE
HELIANTHUS ANNUUS
HIBRIDOS
HYBRIDE
POLIMORFISMO GENETICO
POLYMORPHISME GENETIQUE
ROUMANIE
RUMANIA
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Summary:Eighteen plants of interspecific hybrid H. rigidus (Cass) Desf. x H. annuus L. have been studied in connection with phenotypical characteristics suggesting the introgression of some traits from wilds into cultivated sunflower. Every genotype has been characterized by phenotypical value and by its RFLP profile. Preliminary results are RFLP profiles performed by electrophoresis of total DNA from fresh leaves of every plant and hydrolized by ECO RI and Bam HI restriction enzymes. These profiles showed as well common segments in all descendants as different segments. The last allows the differentiation between the fragments of wild species and cultivated genotypes. The restriction fragments of total DNA have been revealed better, by molecular hybridization using "P" labelled probes K 5 and K 28. Hybridization signals have been more intensive after eight days at -70 degrees C. in a special case
Bibliography:F30
9500101
ISSN:0253-1682