The use of cloned hybridization probes to detect barley yellow dwarf virus infection in tetraploid wheat germplasm

A PAV-like isolate of barley yellow dwarf virus (BYDV) was used in controlled inoculation experiments in the greenhouse to determine the intensity of the autoradiographic signal from positive Northern dot-blot hybridization of leaf sample extract of infected and uninfected plants of tetraploid wheat...

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Bibliographic Details
Main Authors Pace, C. de, Delre, V, Porceddu, E, Casa, R. (Universita della Tuscia, Viterbo (Italy))
Format Conference Proceeding
LanguageEnglish
Published Mexico, DF (Mexico) CIMMYT 1990
Subjects
ADN
CLONE
CLONES
GERMOPLASMA
HIBRIDACION
HYBRIDATION
INFECCION
INFECTION
MATERIEL GENETIQUE
METHODE
METODOS
TETRAPLOIDIA
TETRAPLOIDIE
TRITICUM AESTIVUM
VIRUS ENANISMO AMARILLO DE CEBADA
VIRUS JAUNISSE NANISANTE DE L'ORGE
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Summary:A PAV-like isolate of barley yellow dwarf virus (BYDV) was used in controlled inoculation experiments in the greenhouse to determine the intensity of the autoradiographic signal from positive Northern dot-blot hybridization of leaf sample extract of infected and uninfected plants of tetraploid wheat (Triticum turgidum L.). Radioactive probes gave more consistent results than biotin probes. The proportion of entries with reduced virus content in collections from Ethiopia, Iran, Iraq, and Turkey were 24.3, 28.6, 8.7 and 10.5 %, respectively. No other differential symptoms were expressed on the infested plants in the greenhouse. Therefore, the further evaluation of the tetraploid wheat entries for tolerance should involve (a) field testing and (b) the use of additional criteria to evaluate tolerance to BYDV.
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