The expression of a synthetic cryIA(b) gene in transgenic maize confers resistance to European corn borer

Pest control constitutes a major area of interest for the biotechnology industry. Genes encoding insecticidal proteins have been cloned and they are being introduced in crop plants. At CIBA Agricultural Biotechnology, we have introduced a truncated form of the cryIA(b) gene obtained from Bacillus th...

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Main Authors Estruch, J.J, Carozzi, N.B, Desai, N, Warren, G.W, Duck, N.B, Koziel, M.G
Format Conference Proceeding
LanguageEnglish
Published Mexico, DF (Mexico) CIMMYT 1997
Subjects
BACILLUS THURINGIENSIS
CLONACION
CLONAGE
CLONING
CONTROL DE PLAGAS
GENE
GENES
INSECTE NUISIBLE
INSECTOS DANINOS
LUTTE ANTIRAVAGEUR
PEST CONTROL
PEST INSECTS
PEST RESISTANCE
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
RESISTANCE AUX ORGANISMES NUISIBLES
RESISTENCIA A LAS PLAGAS
TRANSGENIC PLANTS
ZEA MAYS
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Summary:Pest control constitutes a major area of interest for the biotechnology industry. Genes encoding insecticidal proteins have been cloned and they are being introduced in crop plants. At CIBA Agricultural Biotechnology, we have introduced a truncated form of the cryIA(b) gene obtained from Bacillus thuringiensis into an elite line of maize. A synthetic version of the gene was made to increase CryIA(b) protein levels in transgenic maize. The expression of the crylA(b) gene was targeted to the pollen, pith, and green tissues by using appropriate tissue specific promoters. The resulting transgenic maize plants were evaluated for resistance to European corn borer (ECB), Ostrinia nubilalis, under field conditions. Plants with high levels of the crylA(b) protein exhibited excellent resistance to repeated heavy infestations of the pest.
Bibliography:F30
H10
1998000040
ISBN:9686923799
9789686923797