Ploidy level and nuclear DNA content of some accessions of water yam (Dioscorea alata) collected at Save, a district of central Benin

The nuclear DNA content and the ploidy levels of 43 accessions of water yam (Dioscorea alata) collected at Save (a district of central Benin) were determined by both conventional chromosome counts (from root tip cells) and flow cytometry. The great majority (42) of the accessions had 40 chromosomes...

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Published inPlant Genetic Resources Newsletter (IPGRI/FAO) no. 144; pp. 20 - 23
Main Authors Dansi, A. (UNB/FAST, Cotonou (Benin). Laboratoire de genetique), Dainou, O, Agbangla, C, Ahanhanzo, C, Brown, S
Format Publication
LanguageEnglish
Published Rome (Italy) IPGRI/FAO 2005
Subjects
BENIN
CELL COUNTING
CHROMOSOME NUMBER
DIOSCOREA ALATA
DNA
GENETIC RESOURCES
PLANT BREEDING
PLANT COLLECTIONS
POLYPLOIDY
YAMS
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Summary:The nuclear DNA content and the ploidy levels of 43 accessions of water yam (Dioscorea alata) collected at Save (a district of central Benin) were determined by both conventional chromosome counts (from root tip cells) and flow cytometry. The great majority (42) of the accessions had 40 chromosomes and a nuclear DNA content varying from 1.0 pg to 1.26 pg (mean 2C value was 1.07 pg). One accession had 80 chromosomes with a DNA content of 2.12 pg. The ploidy levels (2X and 4X or 4X and 8X) of the analyzed individuals are discussed. These findings have important implications for yam breeding in relation to yam genetic resources
Bibliography:F30
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http://www.bioversityinternational.org/publications/pgrnewsletter/article.asp?lang=en&id_article=4&id_issue=144