Preliminary results on studies of in vivo and in vitro sexual reproduction of Salix viminalis L

In vivo and in vitro self-pollination of whole pistils of some clones of Salix viminalis enabled to obtain mature seeds containing cotyledonary embryos which after the transfer to MS medium developed into wholly formed seedlings. Pollination in vitro of placentae led to abundant pollen germination a...

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Published inDendrobiology Vol. 50
Main Authors Zenkteler, M, Wojciechowicz, M, Bagniewska-Zadworna, A.(Adam Mickiewicz University, Poznan (Poland). Inst. of Experimental Biology), Jezowski, S.(Polish Academy of Sciences, Poznan (Poland). Inst. of Plant Genetics)
Format Journal Article
LanguageEnglish
Subjects
AUTOPOLINIZACION
AUTOPOLLINISATION
CROSS POLLINATION
CULTIVO DE ORGANOS
CULTIVO IN VITRO
CULTURE D'ORGANE
CULTURE IN VITRO
EXPERIMENTACION IN VITRO
EXPERIMENTACION IN VIVO
EXPERIMENTATION IN VITRO
EXPERIMENTATION IN VIVO
GINECEO
GYNECEE
GYNOECIUM
HIBRIDACION INTERESPECIFICA
HYBRIDATION INTERSPECIFIQUE
IN VITRO CULTURE
IN VITRO EXPERIMENTATION
IN VIVO EXPERIMENTATION
INTERSPECIFIC HYBRIDIZATION
ORGAN CULTURE
PLACENTA
POLINIZACION CRUZADA
POLLINISATION CROISEE
POPULUS
POPULUS TOMENTOSA
POPULUS TREMULA
REPRODUCCION SEXUAL
REPRODUCTION SEXUEE
SALIX VIMINALIS
SELF POLLINATION
SEXUAL REPRODUCTION
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Summary:In vivo and in vitro self-pollination of whole pistils of some clones of Salix viminalis enabled to obtain mature seeds containing cotyledonary embryos which after the transfer to MS medium developed into wholly formed seedlings. Pollination in vitro of placentae led to abundant pollen germination and formation of tubes which occasionally they were entering the ovules through micropyle. Fertilized ovules normally developed into germinable seeds. Distant pollination of stigmas in vivo and in vitro with pollen grains of Populus tremula, P. tomentosa, P. lasiocarpa showed the ability of pollen to germinate and to form tubes several hours after pollination. Some tubes penetrated the styles but did not enter into the placenta. When placentae were directly pollinated than pollen germinated abundantly and occasionally pollen tubes were found entering the micropyle. Embryological analysis of those ovules performed 3-5 days after pollination demonstrated the presence of globular embryos with several endosperm nuclei
Bibliography:F63
2005000078
ISSN:1641-1307