Detection of major potato viruses from different potato growing localities of Punjab [Pakistan]

• Main Authors: Ahmed, M, Ahmed, W. (Ayub Agricultural Research Inst., Faisalabad (Pakistan))
• Format: Conference Proceeding
• Language: English
• Published: Islamabad (Pakistan) PSPDP/PARC 1995
• Subjects: IDENTIFICACION; IDENTIFICATION; LUTEOVIRUS DEL ENROLLADO DE PATATA; LUTEOVIRUS ENROULEMENT POMME TERRE; PAKISTAN; POTEXVIRUS X DE LA PATATA; POTEXVIRUS X POMME DE TERRE; POTYVIRUS Y DE LA PATATA; POTYVIRUS Y POMME DE TERRE; SOLANUM TUBEROSUM; TECHNIQUE IMMUNOENZYMATIQUE; TECNICAS INMUNOENZIMATICAS; VIRUS DE LAS PLANTAS; VIRUS DES VEGETAUX

Different potato growing areas of Punjab viz; Jhang, Toba Tek Singh, Chiniot, Okara, Sahiwal, Silakot and Gujranwala were surveyed during the autumn season of 1994 for the detection of major potato viruses i.e. potato leaf roll virus (PLRV), potato virus Y (PVY) and potato virus X (PVX) by the use of enzyme linked immunoabsorbent assay (ELISA). In total 169 fields from seven different localities were visited and 1227 samples were collected which were assayed for the presence of PLRV, PVY and PVX. All 3 tested viruses were detected from all localities surveyed in the Punjab but in different percentage. Percentage of PLRV detected was 12.82 (Jhang), 11.34 (Sialkot), 5.55 (Toba Tek Singh), 5.00 (Gujranwala) 4.41 (Chiniot), 3.63 (Okara) and 3.03 (Sahiwal). PVY was detected in the percentage of 52.77 (Chiniot), 14.37 (Gujranwala), 12.72 (Okara) and 6.81 (Sahiwal). PVX was also detected in the percentage of 20.62 (Gujranwala), 17.94 (Jhang), 16.13 (Okara), 12.87 (Sahiwal), 12.12 (Chiniot), 9.79 (Sialkot) and 2.77 (Toba Tek Singh).