Haematological, biochemical and therapeutic aspects of parturient haemoglobinuria in buffaloes

• Published in: Pakistan journal of zoology Vol. 45; no. 4; pp. 889 - 895
• Main Authors: Mahmood, A. (University of Veterinary and Animal Sciences, Lahore (Pakistan). Dept. of Epidemiology and Public Health), Khan, M.A. (University of Veterinary and Animal Sciences, Lahore (Pakistan). Dept. of Epidemiology and Public Health), Mansur-ud-Din, A. (University of Veterinary and Animal Sciences, Lahore (Pakistan). Dept. of Epidemiology and Public Health), Younus, M. (University of Veterinary and Animal Sciences, Lahore (Pakistan). Dept. of Pathology), Chaudhry, Z.I. (University of Veterinary and Animal Sciences, Lahore (Pakistan). Dept. of Pathology), Ahad, A. (University of Veterinary and Animal Sciences, Lahore (Pakistan). Dept. of Microbiology), Iqbal, H.J. (University of Veterinary and Animal Sciences, Lahore (Pakistan). Dept. of Parasitology), Nasir, M.K. (Government of the Punjab (Pakistan). Dept. of Livestock and Dairy Development), Riaz, M
• Format: Journal Article
• Language: English
• Published: 2015
• Subjects: BIOCHEMISTRY; BLOOD COMPOSITION; CLINICAL TRIALS; DISEASE CONTROL; HAEMOGLOBIN; PARTURIENT PARESIS; PROTEINURIA; WATER BUFFALOES
• ISSN: 0030-9923

A total of thirty buffaloes suffering from parturient haemoglobinuria were selected from district Chakwal during December 2010 - January 2011. Sixty apparently healthy buffaloes of similar description were also selected from the same areas as controls. Blood samples collected with and without anticoagulant (EDTA) were analyzed for various haematological and biochemical parameters. Significantly (P less than 0.05) increased mean corpuscular volume (60.72 plus minus 8.49fL), mean corpuscular haemoglobin (21.46 plus minus 2.52pg), red cell distribution width (16.80 plus minus 1.81%), reticulocytes (1.25 plus minus 0.66%), platelets (208.13 plus minus 85.26x10-3 mul), plateletcrit (0.09 plus minus 0.04%), mean platelet volume (4.99 plus minus 1.85fL), serum alkaline phosphatase (164.20 plus minus 88.95u/L), bilirubin conjugated (0.40 plus minus 0.17mg/dl), bilirubin unconjugated (0.67 plus minus 0.22mg/dl) and bilirubin total (1.07 plus minus 0.39mg/dl) whereas; significantly (P less than 0.05) decreased serum calcium (2.12 plus minus 0.1mmoles/L) and phosphorous (2.67 plus minus 0.79mg/dL) were recorded in haemoglobinuric buffaloes compared to healthy controls. Non-significant (P greater than 0.05) differences were recorded in mean corpuscular haemoglobin concentration, platelet distribution width, serum glucose, cholesterol and copper concentrations between haemoglobinuric and healthy buffaloes. These thirty haemoglobinuric buffaloes were divided into three groups for clinical trial to compare and assess the recovery rates of three different treatment packages. Highest recovery rate (100%) was recorded for combined therapy of sodium acid phosphate and blood transfusion. The recovery rates for combination of sodium acid phosphate with tranexamic acid and tranexamic acid with Novacoc forte injection were 70% and 50%, respectively.