Transformation of tomato (Lycopersicon esculentum Mill.) with Arabidopsis early flowring gene Apetalai (API) through agrobacterium infiltration of ripened fruits

• Published in: Pakistan journal of botany Vol. 40; no. 1; pp. 161 - 173
• Main Authors: Hasan, M. (G.P.G.C. Bannu (Pakistan)), Khan, A.J. (Gomal Univ., D.I. Khan (Pakistan). Gomal Centre of Biotechnology and Biochemistry), Khan, S. (Kohat Univ. of Science and Technology (Pakistan)), Shah, A.H. (Gomal Univ., D.I. Khan (Pakistan). Dept. of Biological Sciences), Khan, A.R. (Gomal Univ., D.I. Khan (Pakistan). Dept. of Biological Sciences), Mirza, B. (Quaid-e-Azam Univ., Islamabad (Pakistan). Faculty of Biological Sciences)
• Format: Journal Article
• Language: English
• Published: 01.02.2008
• Subjects: AGROBACTERIUM; ARABIDOPSIS; GENE EXPRESSION; GENES; GENETIC TRANSFORMATION; GERMINATION; INFILTRATION; INJECTION; LYCOPERSICON ESCULENTUM; PLASMIDS; TOMATOES

In order to develop an easy, rapid and efficient protocol for the genetic transformation of tomato (Lycopersicon esculentum Mill.), without involving tissue culturing, ripened fruits of tomato were infiltrated with Agrobacterium harboring plasmid pROKII GUSint API. The plasmid contains Arabidopsis e...