Transformation of tomato (Lycopersicon esculentum Mill.) with Arabidopsis early flowring gene Apetalai (API) through agrobacterium infiltration of ripened fruits

In order to develop an easy, rapid and efficient protocol for the genetic transformation of tomato (Lycopersicon esculentum Mill.), without involving tissue culturing, ripened fruits of tomato were infiltrated with Agrobacterium harboring plasmid pROKII GUSint API. The plasmid contains Arabidopsis e...

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Published inPakistan journal of botany Vol. 40; no. 1; pp. 161 - 173
Main Authors Hasan, M. (G.P.G.C. Bannu (Pakistan)), Khan, A.J. (Gomal Univ., D.I. Khan (Pakistan). Gomal Centre of Biotechnology and Biochemistry), Khan, S. (Kohat Univ. of Science and Technology (Pakistan)), Shah, A.H. (Gomal Univ., D.I. Khan (Pakistan). Dept. of Biological Sciences), Khan, A.R. (Gomal Univ., D.I. Khan (Pakistan). Dept. of Biological Sciences), Mirza, B. (Quaid-e-Azam Univ., Islamabad (Pakistan). Faculty of Biological Sciences)
Format Journal Article
LanguageEnglish
Published 01.02.2008
Subjects
AGROBACTERIUM
ARABIDOPSIS
GENE EXPRESSION
GENES
GENETIC TRANSFORMATION
GERMINATION
INFILTRATION
INJECTION
LYCOPERSICON ESCULENTUM
PLASMIDS
TOMATOES
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Summary:In order to develop an easy, rapid and efficient protocol for the genetic transformation of tomato (Lycopersicon esculentum Mill.), without involving tissue culturing, ripened fruits of tomato were infiltrated with Agrobacterium harboring plasmid pROKII GUSint API. The plasmid contains Arabidopsis early flowering gene API, GUS reporter gene and NPTII as a selectable marker gene. Both transient and stable GUS expressions were studied with different parameters such as various incubation periods (48, 72 and 96 hours) and tissue type effect. The effects of infiltration on germination of seeds both on selection and non-selection media were studied. The percentage of germination of seeds on non-selection medium was 14% lesser than control, while the germination percentage on selection medium was significantly higher for treated seeds (65-74%) as compared to control (5%), with 48 hours incubation period having maximum (74%).
Bibliography:F30
CATPAK-69954
ISSN:0556-3321