Changes in carbohydrate content and activities of acid invertase,sucrose synthase and sucrose phosphate synthase in broccoli duringshort term storage at low temperature

• Published in: Asian Journal of Plant Sciences (Pakistan) Vol. 3; no. 4
• Main Authors: Pramanik, B.K, Matsui, T, Suzuki, H, Kosugi, Y.,Kagawa Univ.(Japan). Dept. of Bioresource Production
• Format: Journal Article
• Language: English
• Published: 2004
• Subjects: BROCCOLI; COLD STORAGE; POSTHARVEST PHYSIOLOGY; VARIETIES
• ISSN: 1682-3974

This study investigated the changes in carbohydrate content andactivities of invertase, sucrose syntheses and sucrose phosphatesyntheses in two broccoli cultivar (Brassica oleracea L. Cvs. `Hartland'and `Sairin') stored at 5 degree C. Respiration rate rapidly declinedafter 1 day of storage and gradually decreased at the end of thestorage period. Color did not change significantly at the end of thestorage period. In both cultivars and portions, the invertase activityincreased gradually at the end of the storage time. The acid invertasein cell wall- bound fraction (CWBF) showed a higher activity than thatin soluble fraction (SF). Again, the branchlets showed a significantlyhigher invertase activity than that of florets. In both cultivars,sucrose syntheses (SS) activity gradually increased in the floretswith some fluctuations in the branchlets at the end of the storagetime. Sucrose phosphate syntheses activity (SPS) activity did notchange markedly in any portion of the two cultivars at the end of thestorage period. Sucrose content gradually decreased in both portionsof the two cultivars with time. It was negatively correlated with theacid invertase and SS activities in both portions accounting well forthe relation between the substrate and the activity. Fructose andglucose content were higher than sucrose in the florets as well asbranchlets in both cultivars.