BrCIPK1 gene enhances multiple abiotic stress tolerance in rice

• Published in: Philippine Journal of Crop Science (Philippines) Vol. 38; no. 1
• Main Authors: Abdula, S.E, Lee, H.J, Ryu, H, Cho, Y.G
• Format: Journal Article
• Language: English
• Published: 01.03.2013
• Subjects: BIOSINTESIS; BIOSYNTHESE; BIOSYNTHESIS; ESTRES; EXPRESION GENICA; EXPRESSION DES GENES; GENE; GENE EXPRESSION; GENES; http://www.fao.org/aos/agrovoc#c_27527; http://www.fao.org/aos/agrovoc#c_3214; http://www.fao.org/aos/agrovoc#c_34283; http://www.fao.org/aos/agrovoc#c_37740; http://www.fao.org/aos/agrovoc#c_5438; http://www.fao.org/aos/agrovoc#c_7452; http://www.fao.org/aos/agrovoc#c_928; ORGANISME TRANSGENIQUE; ORYZA SATIVA; STRESS; TOLERANCE; TOLERANCIA; TRANSGENICOS; TRANSGENICS
• ISSN: 0115-463X

A cDNA clone encoding CBL-interacting protein kinase 1 (CIPK1) was isolated from Chinese cabbage seedlings and introduced into rice via Agrobacterium transformation. The gene, BrCIPK1 consisted of 1,982 bp long with 216 bp of the 5'-untranslated region (UTR), 1,509 bp of the coding region and 257 bp of the 3'-UTR. It is highly conserved (BL-interacting module with absolutely conserved domain among the 15 amino acid NAF domain of the 15 related genes. Southern blot analysis showed a single copy number. BrCIPK1 gene was localized in the cytoplasm and peripheral region in the plant cell which in highly expressed in seedling of rice and in the shoot and pistil of Arabidopsis. Analyses of gene expression on Ubi-1::BrCIPK1 rice lines was differentially accumulated by cold, salinity and drought, indicating its biological roles in the multiple stress response pathways in plants. Consistent with stress biosynthesis, was upregulated by the BrCIPK1 during abiotic stresses resulting to improved accumulation of proline. Further, yeast two hybrid analysis showed the BrCIPK1 was interacted with two rice calcineurin -B-like proteins (OS CBL 1 and 8). The interaction of BrCIPK1 with OsCBL 1 + 8 along with the regulation of P5CS1 explained the enhanced tolerance to abiotic stress of the transgenic rice. Results suggests that this gene could be used in the development of rice varieties with enhanced tolerance to abiotic stresses.