Characterization of the antimicrobial substances produced by Bacillus sp. B-1 isolated from culture pond

• Published in: Journal of ISSAAS Vol. 16; no. 1
• Main Authors: Pungpang, S, Areechon, N, Wilaipun, P., Kasetsart Univ., Kamphaeng Saen Campus, Nakhon Pathom 73140 (Thailand). Center for Agricultural Biotechnology
• Format: Journal Article
• Language: English
• Published: 01.06.2010
• Subjects: AEROMONAS; AISLAMIENTO; ANTIMICROBIALS; ANTIMICROBIANOS; ANTIMICROBIEN; BACILLUS; BACILO; BACTERIOCINAS; BACTERIOCINE; BACTERIOCINS; BIOLOGICAL PROPERTIES; CHEMICOPHYSICAL PROPERTIES; CHLORURE DE SODIUM; CLORURO SODICO; ESCHERICHIA COLI; ESTANQUES; ETANG; http://www.fao.org/aos/agrovoc#c_1501; http://www.fao.org/aos/agrovoc#c_1521; http://www.fao.org/aos/agrovoc#c_157; http://www.fao.org/aos/agrovoc#c_16429; http://www.fao.org/aos/agrovoc#c_26553; http://www.fao.org/aos/agrovoc#c_33700; http://www.fao.org/aos/agrovoc#c_33944; http://www.fao.org/aos/agrovoc#c_37744; http://www.fao.org/aos/agrovoc#c_509; http://www.fao.org/aos/agrovoc#c_5753; http://www.fao.org/aos/agrovoc#c_6105; http://www.fao.org/aos/agrovoc#c_7146; http://www.fao.org/aos/agrovoc#c_760; ISOLATION; ISOLEMENT; LISTERIA MONOCYTOGENES; PONDS; PROPIEDADES BIOLOGICAS; PROPIEDADES FISICOQUIMICAS; PROPRIETE BIOLOGIQUE; PROPRIETE PHYSICOCHIMIQUE; SODIUM CHLORIDE; STAPHYLOCOCCUS AUREAS; STAPHYLOCOCCUS AUREUS
• ISSN: 0859-3132

Genus Bacillus produces many antimicrobial peptides of varying structures such as bacteriocins, sublancin and subtilosin. Although bacteriocins active against a varied bacteria including important pathogens such as Listeria monocytogenes, Staphylococcus aureus and Streptococcus sp. The aim of this study was preliminary characterization of antimicrobial substances especially biological and physical properties such as high temperature, pH, sensitivity of proteolysis enzymes and sodium chloride (NaCl). The antimicrobial activity was determined by bacteriocin activity assay by the application of the critical dilution method (agar well diffusion) described by Schillinger et al. (1989). Bacillus sp. B-1 produce antimicrobial peptides with properties of inhibition effects on Streptococcus agalactiae AQSA 001, Aeromonas hydrophyla AQAS 001, Staphylococcus aureus ATCC 12600, Escherichia coli JM 109, Listeria innocua ATCC 33090, Micrococcus luteus IFO 12708, Bacillus circulans JCM 2504 and B. coagulans JCM 2257 and especially L. innocua ATCC 33090 which yielded the highest sensitivities of indicator strain (320 AU/ml). The cell-free nuetralized supernatant of Bacillus sp. B-1 were stable at 100 deg C during a period of 20 minutes (20 AU/ml) and maximum activity of pH 4.0, 6.0, 8.0 and 9.0 at 4 deg C (320 AU/ml) and maximum activities of pH 2.0, 4.0 and 6.0 at 100 deg C for 60 minutes were 80 AU/ml. The antimicrobial substances were sensitive to the proteolytic properties of a-chrymotrypsin, trypsin, proteinase K and protease and exhibited stable qualities at 6, 12 and 15% sodium chloride.