Genetic characterization of near-isogenic lines for blast resistance with indica-type line, IR49830-7-1-22 genetic background
Eight kinds of near isogenic lines (NILs) were developed for blast resistance with the genetic background of indica-type line IR49830-7-1-22 by using recurrent backcross breeding. Eight different kinds of single blast resistance genes - PiK, Pi2, Pi2-5, Pita-2, Pi3(t), Pi5(t), and Pi9(t)- were intro...
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Published in | Journal of Tropical Plant Pathology (Philippines) Vol. 40; no. 1-2 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.01.2004
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Subjects | |
Online Access | Get more information |
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Summary: | Eight kinds of near isogenic lines (NILs) were developed for blast resistance with the genetic background of indica-type line IR49830-7-1-22 by using recurrent backcross breeding. Eight different kinds of single blast resistance genes - PiK, Pi2, Pi2-5, Pita-2, Pi3(t), Pi5(t), and Pi9(t)- were introduced in each NIL using selected Philippine isolates of the blast fungus Pyricularia grisea Sacc. The genotypes of the recurrent parent, IR49830-7-1-22, and each NIL were confirmed by differential system and allelism test. This was carried out by inoculating F2 plant populations derived from the crosses with monogenic lines and NILs with CO 39 genetic background as differentials with avirulent and virulent blast isolates. Four genes - Pia, Pib, Pik-5, and Pita- were found in IR49830-7-1-22, and the target gene was introduced in each NIL. These NILs were designated as IRBLk-Ku/RL, IRBLz-Fu/RL, IRBLz5-CA/RL, IRBLTa2-Pi/RL, IRBL3-CP1/RL, IRBL5-M/RL, IRBL7-M/RL and IRBL9-W/RL. |
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Bibliography: | F30 2007000489 H20 |
ISSN: | 0115-0804 |