Identification and study of some physiological characteristics of the rotted roots of apple, at Zacatlan, Puebla

From rotted roots of apple trees a fungus was isolated and cultured on synthetic, semisynthetic and natural media. The media that favored vegetative growth of the fungus were: Malt + Thiamin-Agar at 25 deg. C. and alternate conditions of fluorescent light (12 hours) and darkness (12 hours). PDA. Oat...

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Bibliographic Details
Published inAgrociencia (Mexico) no. 77
Main Authors Carrillo Fonseca, C.L, Romero C, S
Format Journal Article
LanguageSpanish
Published 1989
Subjects
CHAMPIGNON
FACTEUR DU MILIEU
IDENTIFICATION
MALUS PUMILA
PUEBLA
ROSELLINIA
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Summary:From rotted roots of apple trees a fungus was isolated and cultured on synthetic, semisynthetic and natural media. The media that favored vegetative growth of the fungus were: Malt + Thiamin-Agar at 25 deg. C. and alternate conditions of fluorescent light (12 hours) and darkness (12 hours). PDA. Oat-Agar and V8-Juice-Agar. The fungus produced asexual fructifications (synnemata) on infusion of apple tree sprouts-Agar, under continuous light and alternate conditions of fluorescent light and darkness. On Tomato-Agar and Oat-Agar media asexual fructifications also were formed. In moist chamber synnemata sprouted continuously until the diseased roots underwent disintegration. It was not possible to obtain the perithecial stage on the media utilized or to observe it on apple trees at the zone of study. According to the morphological characteristics of the mycelium and synnemata of the fungus, it may be concluded that it corresponds to the species Dematophora necatrix (Hart.) Berlese. De plantas de manzano con pudricion blanca de la raiz se aislo un hongo, el que se cultivo en medios sinteticos, semisinteticos y naturales bajo diferentes condiciones ambientales. Los medios que estimularon el desarrollo vegetativo del hongo fueron: malta mas tiamina a 25 grados C en condiciones alternadas de luz y oscuridad;PDA, avena-agar y jugo V8-agar. La fase asexual del hongo (sinematica) se obtuvo en infusion de hijuelos de manzano agar, bajo luz continua y alternancia de luz-oscuridad. Asimismo, dicha fase se aprecio en los medios: tomate y avena-agar. En camara humeda se formaron los sinemas hasta la descomposicion total de la raiz. No fue posible observar la fase perfecta en medio de cultivo, ni en los arboles enfermos de la zona bajo estudio. De acuerdo a las caracteristicas morfologicas del micelio y de la fase asexual producida por el hongo se concluye que corresponde a la especie Dematophora necatrix (Hart.) Berlese.
Bibliography:H20
9200314
ISSN:0185-0288