Immuno-enhancing Effect of Seed Extracts on a RAW 264.7 Macrophage Cell Line

• Published in: Han'guk Sikp'um Yŏngyang Kwahakhoe chi Vol. 41; no. 12
• Main Authors: Yu, A.R., Korea Food Research Institute, Seongnam, Republic of Korea, Park, H.Y., Korea Food Research Institute, Seongnam, Republic of Korea, Kim, Y.S., Korea Food Research Institute, Seongnam, Republic of Korea, Ha, S.K., Korea Food Research Institute, Seongnam, Republic of Korea, Hong, H.D., Korea Food Research Institute, Seongnam, Republic of Korea, Choi, H.D., Korea Food Research Institute, Seongnam, Republic of Korea
• Format: Journal Article
• Language: Korean
• Published: 01.12.2012
• Subjects: CITOQUINAS; CYTOKINE; CYTOKINES; immune-enhancing; NITRIC OXIDE; OXIDO NITRICO; OXYDE NITRIQUE; RAW 264.7 cell; SEED; SEMENCE; SEMILLAS
• ISSN: 1226-3311

In this study, the immuno-enhancing activity of seed extracts were studied on the macrophage cell lines. We examined the effect of nine seed extracts on nitric oxide (NO) production in RAW 264.7 cells and selected four highly-effective seed candidates (Fagopyrum esculentum, Taraxacum platycarpum, Impatiens balsamina, Helianthus annuus) for further immune-related studies. The effects of the four seed extracts on the production of immune-related cytokines in the RAW 264.7 macrophage cell line and proliferation of Molt-4 as a T cell line were investigated. The secretion of NO from the RAW 264.7 cells was increased up to 39 μM by adding the seed extracts (25 ㎍/mL) compared to the control. Also, the secretion of tumor necrosis factor-alpha (TNF-α) was also increased up to 32 times by adding the seed extracts (25 ㎍/mL). Secretion of cytokines such as interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and interleukin-10 (IL-10) was also increased and induced the proliferation of T cells compared to the control. In conclusion, these results suggest that four seed extracts provide beneficial immuno-enhancing effects for human health.