Study on Antioxidant Effects of Fractional Extracts from Ligularia stenocephala Leaves

• Published in: Han'guk Sikp'um Yŏngyang Kwahakhoe chi Vol. 41; no. 9
• Main Authors: Kim, K.H., Chungnam National University, Daejeon, Republic of Korea, Kim, N.Y., Joongbu University, Kumsan, Republic of Korea, Kim, S.H., Joongbu University, Kumsan, Republic of Korea, Han, I.A., Chungnam National University, Daejeon, Republic of Korea, Yook, H.S., Chungnam National University, Daejeon, Republic of Korea
• Format: Journal Article
• Language: Korean
• Published: 01.09.2012
• Subjects: ANTIMICROBIALS; ANTIMICROBIANOS; ANTIMICROBIEN; ANTIOXIDANTES; ANTIOXIDANTS; ANTIOXYDANT; Ligularia stenocephala leaves
• ISSN: 1226-3311

Ligularia stenocephala leaves were subjected to extraction by using several solvents with different polarities, which were then investigated for their antioxidant and antimicrobial activities. Extraction yield of ethanol extract of Ligularia stenocephala was 21.36%. The water fraction showed the highest extraction yield of 60.35%, followed by the n-butanol, n-hexane, and ethyl acetate fractions. Total polyphenolic content was the highest (254.00 mg/g GAE) in the ethyl acetate fraction of Ligularia stenocephala extract. The ethyl acetate fraction had IC∧50 values of 0.28 mg/mL for DPPH radical scavenging, and 96.67±0.09% for ABTS radical scavenging activity. Further, FRAP value was significantly higher in the ethyl acetate fraction. The ethyl acetate fraction showed antimicrobial activities against B. cereus, and the diameter of the zone of inhibition was 10.2 mm at 5 mg/disc. These results suggest that the ethyl acetate fraction of Ligularia stenocephala possesses the antimicrobial activities against B. cereus, and its high antioxidant activity could be applicable to food additives and as a natural cosmetic ingredient.