Primary Culture of Chicken Tracheal Epithelial Cells and Study on Those Characters for Recombinant Virus Infection

• Published in: Korean journal of poultry science Vol. 40; no. 4
• Main Authors: Park, M.N., RDA, Suwon, Republic of Korea, Jang, H.J., RDA, Suwon, Republic of Korea, Keum, D.H., RDA, Suwon, Republic of Korea, Choi, J.A., RDA, Suwon, Republic of Korea, Yoo, J.G., RDA, Suwon, Republic of Korea, Byun, S.J., RDA, Suwon, Republic of Korea, Park, J.J., RDA, Suwon, Republic of Korea, Ji, J.Y., RDA, Suwon, Republic of Korea, Lee, K.T., RDA, Suwon, Republic of Korea, Kim, T.H., RDA, Suwon, Republic of Korea, Lee, H.J., RDA, Suwon, Republic of Korea
• Format: Journal Article
• Language: Korean
• Published: 01.12.2013
• Subjects: CHICKENS; POLLO; POULET; tracheal epithelial cell, primary culture, viral respiratory diseases
• ISSN: 1225-6625

Tracheal epithelial cells (TECs) are an important tool for studies of viral respiratory diseases. Primary TECs have been cultured from human, mouse and hamster. It is also necessary to diagnose viral respiratory disease and reveal infection mechanisms in chicken. In this study, we isolated tracheal epithelial layers from tracheal of 20-day-old chicks and cultured primary TECs from the isolated layers. Ciliated cells which were a typical morphology of TECs were observed in cultured primary TECs and maintained until cell passage 5 (15 to 20 days). When we analyzed expression patterns of epithelial marker genes (retinoic acid responder, FGF-binding protein, virus activating protease (VAP) in TECs compared to immortalized chicken embryonic fibroblast cell line (DF-1), all the marker genes are highly expressed in TECs than in DF-1. When TECs were cultured with 0.1 and 1 MOI of ND virus (rNDV-GFP strain) to test the susceptibility of TECs for ND virus, 12.6% and 48.2% of the incubated TECs were infected respectively. In addition, when DF-1 was incubated with 1 MOI of ND virus, the virus infection rate of DF-1 was three times lower than the virus infection rate of TECs. These data could contribute to study infection mechanisms of viral respiratory diseases and control them in chicken.