Genotypic Identification in Commercial Strains of Pleurotus ostreatus based on AKLP and VCGs

We already reported four groups which contains some similar strains on URP-PCR in the previous paper. The objective of this study was to confirm those strains by the amplified fragment length polymorphism and vegetaive compatibility group. AFLP analysis showed specific DNA bands only in the result o...

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Bibliographic Details
Published inHangug gynnhaghoi ji Vol. 41; no. 1
Main Authors Seo, K.I., RDA, Eumseong, Republic of Korea, Yoo, Y.B., RDA, Eumseong, Republic of Korea, Jang, K.Y., RDA, Eumseong, Republic of Korea, Shin, P.G., RDA, Eumseong, Republic of Korea, Oh, Y.L., RDA, Eumseong, Republic of Korea, Kim, K.H., Konkuk University, Seoul, Republic of Korea, Kong, W.S., RDA, Eumseong, Republic of Korea
Format Journal Article
LanguageKorean
Published 01.03.2013
Subjects
AFLP,phylogenetic relationship,strain identification, VCG
PLEUROTUS OSTREATUS
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Summary:We already reported four groups which contains some similar strains on URP-PCR in the previous paper. The objective of this study was to confirm those strains by the amplified fragment length polymorphism and vegetaive compatibility group. AFLP analysis showed specific DNA bands only in the result of P+ AG/M +AAG and P+ GT/M+ATG primer combinations out of eight different combinations. The AFLP promers produced a total of 330 fragments between 80 and 1000 bp in length for 31 pleurotus ostreatus strains. At a genetic similarity of 0.96, the UPGMA analysis separated the siolates into four distinct clusters.
Bibliography:A50
ISSN:0253-651X
2383-5249