Effect of Glycerol and Ethylene Glycol on Post-Thawed Sperm Function in Jeju Horse

• Published in: Reproductive & developmental biology Vol. 36; no. 3
• Main Authors: Oh, S.A., National Institute of Animal Science, RDA, Jeju, Republic of Korea, Ko, M.H., National Institute of Animal Science, RDA, Jeju, Republic of Korea, Ko, M.S., National Institute of Animal Science, RDA, Jeju, Republic of Korea, Lee, C.E., National Institute of Animal Science, RDA, Jeju, Republic of Korea, Park, Y.S., National Institute of Animal Science, RDA, Jeju, Republic of Korea, Kang, T.Y., Jeju National University, Jeju, Republic of Korea, Ko, J.H., Woori Equine Hospital, Jeju, Republic of Korea, Cho, W.M., National Institute of Animal Science, RDA, Jeju, Republic of Korea
• Format: Journal Article
• Language: Korean
• Published: 01.09.2012
• Subjects: ETHYLENE GLYCOL; ETILENOGLICOL; GLICEROL; GLYCEROL; JeJu horse; Semen freezing
• ISSN: 1738-2432

Cryopreservation induces sublethal damage to the spermatozoa, which leads to their reduced fertile life. This study was designed to determine effect of glycerol and ethylene glycol as cryoprotectant in extender on improve the freezability of Jeju horse semen. The semen was cryopreserved with glucose-EDTA extender containing each 5% glycerol, 5% ethylene glycol, 8% glycerol or 8% ethylene glycol, respectively. Post-thawed sperm were evaluated motility, viability, Membrane integrity and acrosome integrity. Post-thawed sperm motility were not significantly differences among treatments. However, sperm viability were significantly higher (p less than 0.05) in 8% glycerol (39.85%±11.41) than in 5% glycerol treatment (18.08%±1.61). In membrane integrity, swelling sperm ratio was significantly higher (p less than 0.05) in 8% glycerol (34.12%±11.02) than other treatments. In the percentage of capacitated sperm assessed by CTC staining, F pattern was significantly higher in 8% ethylene glycol than 5% glycerol and 5% ethylene glycol (p less than 0.05). B pattern ratio was significantly increased in 5% ethylene glycol compared with 8% glcerol and 8% ethylene glycol (p less than 0.05). Moreover, 8% ethylene glycol treatment was significantly decreased AR pattern ratio compared with other treatments (p less than 0.05). It is concluded that treatment of 8% glycerol was improved the sperm viability and 8% ethylene glycol was improved the sperm ascrosome integrity after thawing. However, they were not significantly difference between 8% glycerol and 8% ethylene glycol on post-thawed sperm viability. Therefore, 8% ethylene glycol was more effective sperm cryoprotectant than 8% glycerol in Jeju Horse.