Immune activation of Jeju water containing vanadium on peripheral immunocytes, of Low dose gamma rays-irradiated mice

• Published in: Korean Journal of Veterinary Public Health Vol. 35; no. 1
• Main Authors: Ha, D.B., Jeju National University, Jeju, Republic of Korea, Kim, M.J., Korea Basic Science Institute, Jeju, Republic of Korea, Joo, H.J., Jeju National University, Jeju, Republic of Korea, Cho, J.H., Jeju National University, Jeju, Republic of Korea, Bing, S.J., Jeju National University, Jeju, Republic of Korea, Lim, Y.K., Jeju National University, Jeju, Republic of Korea, Hyun, J.W., Jeju National University, Jeju, Republic of Korea, Jee, Y.H., Jeju National University, Jeju, Republic of Korea
• Format: Journal Article
• Language: Korean
• Published: 01.03.2011
• Subjects: Immune Activation; Jeju Water; VANADIO; VANADIUM
• ISSN: 1225-1739

Vanadium, a dietary micronutrient, has been reported to present interesting biological and pharmacological properties, including superoxide and nitric oxide scavenging effects. Low-dose ionizing radiation (LDR) is known to damage DNA and cause apoptosis of peripheral immunocytes by producing reactive oxygen species (ROS). The aim of this study was to elucidate the capacity of immune activation of Jeju water containing vanadium on immunosuppression caused by LDR. We examined the ROS production, DNA damage, cell apoptosis and proliferation of peripheral immunocytes in irradiated mice drinking different concentrations for 90 days; V∧0 (vanadium 0㎍/L, control), V₁ (vanadium 15~20㎍/ L) and V₂ (vanadium 20∼25㎍/L). Compared to V∧0 control where level of ROS showed tendency to increase, the ROS production was attenuated in peripheral immunocytes of irradiated mice drinking V₁ and V₂. DNA damage of peripheral immunocytes triggered by LDR significantly increased in mice drinking V∧0 compared to non-irradiated control, whereas V₁ and V₂ dramatically induced remission of DNA damage. On the observation of apoptosis of peripheral immunocytes, V₁ and V₂ showed the potency to reduce the number of apoptotic cells. On the other hand irradiated mice drinking V∧0 exhibited raised number of apoptotic cells. From the results obtained, we speculated that Jeju water containing vanadium (V₁ and V₂) has a potential role in decreasing DNA damage and apoptosis of immune cell by inhibiting ROS production. Consistent with this, Jeju water containing vanadium (V₁ and V₂) exhibits a capacity to enhance cell proliferation of peripheral immunocytes, which is suppressed by LDR as shown in V∧0 control. Collectively, Jeju water containing vanadium reduced DNA damage and apoptosis and induced the stimulatory potential on immunocytes. Theseresults suggest that Jeju water containing vanadium sustained immune activities under immunosuppression caused by LDR.