Fungal pathogen protection in transgenic lettuce by expression of a apoptosis related Bcl-2 gene

Transgenic lettuce plants were successfully obtained from hypocotyl explants inoculated with Agrobacterium tumefaciens, which harbored a binary vector plasmid with Bcl-2 gene, related to apoptosis. After culture and selection on MS medium a number of kanamycin-resistant plantlets were regenerated. P...

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Bibliographic Details
Published inJournal of Plant Biotechnology Vol. 38; no. 3
Main Authors Seo, K.S., Kyungpook National University, Sangju, Republic of Korea, Min, B.W., Kyungpook National University, Sangju, Republic of Korea
Format Journal Article
LanguageKorean
Published 01.09.2011
Subjects
APOPTOSE
APOPTOSIS
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Summary:Transgenic lettuce plants were successfully obtained from hypocotyl explants inoculated with Agrobacterium tumefaciens, which harbored a binary vector plasmid with Bcl-2 gene, related to apoptosis. After culture and selection on MS medium a number of kanamycin-resistant plantlets were regenerated. Polymerase chain reaction, Southern blot analysis and Northern blot analysis were used to identify and characterize the transgenic plants with the integrated Bcl-2 gene. Over 100 transgenic plants have been established in soil and flowered in the greenhouse. T1 progeny of 100 transgenic lettuce inbred lines were inoculated with Sclerotinia sclerotiorum. Expression of the Bcl-2 peptide in transgenic lettuce plants provides high levels of field resistance against Sclerotinia sclerotiorum, causal agent of the agronomically important fungal disease of lettuce.
Bibliography:F30
2012002755
ISSN:1598-6365
2384-1397