Development of Suhan Strain-specific SCAR Marker in Pleurotus ostreatus

In this study, 81 commercial strains of Pleurotus species cultivated in South Korea were analyzed with randomly amplified polymorphic DNA (RAPD) technique. Sequence characterized amplified region (SCAR) markers were developed by designing from one RAPD polymorhic band specific to Suhan strain. The S...

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Published inHangug gynnhaghoi ji Vol. 39; no. 1
Main Authors Seo, K.I., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea, Jang, K.Y., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea, Yoo, Y.B., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea, Park, S.Y., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea, Kim, K.H., Konkuk University, Seoul, Republic of Korea, Kong, W.S., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea
Format Journal Article
LanguageKorean
Published 01.04.2011
Subjects
PLEUROTUS OSTREATUS
RAPD
SCAR marker
Specific primers
Suhan strain
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Summary:In this study, 81 commercial strains of Pleurotus species cultivated in South Korea were analyzed with randomly amplified polymorphic DNA (RAPD) technique. Sequence characterized amplified region (SCAR) markers were developed by designing from one RAPD polymorhic band specific to Suhan strain. The SCAR primer pair 'S-OPA13-1' amplified a 590-bp fragment in the varieties originated from Suhan strain. The Blast search of S-OPA13-1 showed high homology to the POMFBO1 P. ostreatus cDNA clone MFB02-A05 and Laccaria bicolor S238N-H82. The results showed that this SCAR marker can clearly distinguish Suhan strains from Pleurotus spp.
Bibliography:2012002397
A50
ISSN:0253-651X
2383-5249