Cold-Sensitive Growth of Bacillus subtilis Mutants Deleted for Putative DEAD-Box RNA Helicase Genes

• Published in: The Korean Journal of Microbiology Vol. 46; no. 3
• Main Authors: Oh, E.H., Pai Chai University, Daejeon, Republic of Korea, Lee, S.S., Pai Chai University, Daejeon, Republic of Korea
• Format: Journal Article
• Language: Korean
• Published: 01.09.2010
• Subjects: BACILLUS SUBTILIS; cold-sensitive; DEAD-box RNA helicase; RIBOSOMAS; RIBOSOME; RIBOSOMES
• ISSN: 0440-2413

Four genes (yqfR, yfmL, ydbR, deaD) were identified as putative DEAD-box RNA helicase genes in the genomic sequence of Bacillus subtilis by homology search. To understand the function of these genes, each of the genes was deleted and the constructed strains were tested for their growth charateristics at different temperatures. The growth rate of ydbR deletion mutant (T∧d=53 min) was a little bit reduced at 37℃ as compared to that of wild type strain (CU1065). But the growth rate of other three (yqfR, yfmL, deaD) deletion mutants (T∧d=30-40 min) is nearly equal to the growth rate of wild type (T∧d=32 min). On the other hands, the growth rate of deletion mutants were reduced at 22℃ in order of yqfR (T∧d=151 min), yfmL (Td=214 min), ydbR (T∧d=343 min), which showed cold-sensitive phenotype. The deletion mutant of deaD (T∧d=109 min) grew equally as compared to the growth rate (T∧d=102 min) of the wild type at 22℃ and did not show cold-sensitive growth. Double, triple and quadruple deletion mutants of these genes were constructed, and growth rate of these mutants were measured at various temperature conditions (22℃, 37℃, 42℃) using LB broth. Multiple deletion mutations showed more severe cold-sensitive growth than single deletion mutations, and double deletion of ydbR and yfmL (T∧d=984 min) showed most cold-sensitive growth than any other double mutants. Such a cold-sensitive growth of these mutations is quite similar to the result of csdA or srmB deletion in E. coli and suggested that physiological role of ydbR and yfmL is related with ribosome assembly.