Selection of High Efficient Enzyme for Protoplasts Isolation from Mushrooms

This study was carried out to select cell wall degrading enzymes for maximizing protoplast yield from Basidiomycetes. The protoplasts were released from spore suspension, mycelia cultured on cellophane membrane, and homogenized mycelia of Flammulina velutipes using commercial cell wall degrading enz...

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Bibliographic Details
Published inHangug gynnhaghoi ji Vol. 38; no. 1
Main Authors Kim, J.K., Hankyong National University, Ansung, Republic of Korea, Kim, J.H., Hankyong National University, Ansung, Republic of Korea, Kong, W.S., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea, Kang, H.W., Hankyong National University, Ansung, Republic of Korea
Format Journal Article
LanguageKorean
Published 01.06.2010
Subjects
BASIDIOMYCOTINA
Cell wall degradation enzymes
PROTOPLASTE
PROTOPLASTOS
PROTOPLASTS
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Summary:This study was carried out to select cell wall degrading enzymes for maximizing protoplast yield from Basidiomycetes. The protoplasts were released from spore suspension, mycelia cultured on cellophane membrane, and homogenized mycelia of Flammulina velutipes using commercial cell wall degrading enzymes. The highest yield of protoplasts was obtained from the homogenized mycelia treated with the enzyme combination of Glucanex∨R 200G and cellulase onozuka R-10. The protocol was also available for Pleurotus ostreatus, P. eryngii, and Hypsizygus marmoreus.
Bibliography:2011000541
A50
ISSN:0253-651X
2383-5249