Effect of Making a Hole in Zona Pellucida by Laser on Hatching of Frozen-thawed ICR Mouse Embryos

This study was performed to investigate the effect of laser-assisted hole in the zona pellucida (ZP) of frozen-thawed ICR mouse embryos on the process of hatching that is critical for expanded blastocysts to implant into endometrium. Vitrification medium, composed of ethylene glycol and sucrose supp...

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Bibliographic Details
Published inJournal of Embryo Transfer Vol. 23; no. 1
Main Author Yong, H.Y. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: yongh@chungbuk.ac.kr
Format Journal Article
LanguageKorean
Published 01.03.2008
Subjects
BIOLOGICAL PRESERVATION
CONGELACION
CONGELATION
CONSERVACION BIOLOGICA
CONSERVATION BIOLOGIQUE
ECLOSION
embryo
FREEZING
HATCHING
LASER
LASERS
MICE
RATON
SOURIS
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Summary:This study was performed to investigate the effect of laser-assisted hole in the zona pellucida (ZP) of frozen-thawed ICR mouse embryos on the process of hatching that is critical for expanded blastocysts to implant into endometrium. Vitrification medium, composed of ethylene glycol and sucrose supplemented with 7.5% (w/v) PVP, was used to freeze 2~4 cell stage embryos recovered from oviducts of superovulated and mated female mice before storing them in LM₂. Right after thawing them, a laser beam was shot to make a hole in ZP followed by culturing in KSOM for 96~120 hr and examining development to blastocyst and hatching every 12 hr. Laser-treated embryos showed significantly higher hatching rate compared to control (92.9% vs. 22.1%, p less than 0.05). From around Day 4, blastocysts developed from laser-treated embryos started hatching while the blastocysts of control group failed to hatch showing a lot of shrinkage. This study shows that a laser-assisted hole in ZP improves the hatching rate of blastocysts developed from frozen-thawed, in vitro cultured ICR mouse embryos.
Bibliography:2009003365
L53
ISSN:1225-4991