Characterization of Bacillus thuringiensis subsp. tohokuensis CAB167 Isolate against Mosquito Larva
Eight Bacillus thuringiensis strains activated against mosquito larva were compared their characterization. Spherical-shaped parasporal inclusion of B. thuringiensis subsp. tohokuensis CAB167 was observed by phase-contrast microscopy and scanning electron microscopy. LC∧50 values of B. thuringiensis...
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Published in | Korean Journal of Applied Entomology Vol. 47; no. 4 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | Korean |
Published |
01.12.2008
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Subjects | |
Online Access | Get more information |
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Summary: | Eight Bacillus thuringiensis strains activated against mosquito larva were compared their characterization. Spherical-shaped parasporal inclusion of B. thuringiensis subsp. tohokuensis CAB167 was observed by phase-contrast microscopy and scanning electron microscopy. LC∧50 values of B. thuringiensis subsp. tohokuensis CAB167 against Culex pipiens molestus, Culex pipiens pallens, and Aedes aegyti were 173, 190 and 580 ng/ml, respectively. B. thuringiensis subsp. tohokuensis CAB167 had a parasporal inclusion containing 4 major protein components, for example, 135, 80, 49 and 28-kDa by SDS-PAGE. Otherwise, after trypsin digestion of parasporal inclusion, SDS-PAGE was showed new protease-resistant peptides at 72 and 63-kDa. Activated toxins of isolated CAB167 were different from other reference strains on a serological by immuno-diffusion test. |
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Bibliography: | A50 2009002086 |
ISSN: | 1225-0171 |