cSNP Identification and Genotyping from C4B and BAT2 Assigned to the SLA Class Ⅲ Region

C4B and BAT2, assigned to the SLA class Ⅲ region, were recently reported on relation with human diseases. The primers for RT-PCR and RACE-PCR for CDS analysis of these genes of pig were designed by aligning the CDSs of humans and mice from GenBank. After we amplified and sequenced with these primers...

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Published inJournal of Animal Science and Technology Vol. 49; no. 5
Main Authors Kim, J.H. (Gyeongsang National University, Jinju, Republic of Korea), Lim, H.T. (Gyeongsang National University, Jinju, Republic of Korea), Seo, B.Y. (Gyeongsang National University, Jinju, Republic of Korea), Lee, S.H. (Gyeongsang National University, Jinju, Republic of Korea), Lee, J.B. (Gyeongsang National University, Jinju, Republic of Korea), Yoo, C.K. (Gyeongsang National University, Jinju, Republic of Korea), Jung, E.J. (Gyeongsang National University, Jinju, Republic of Korea), Jeon, J.T. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: jtjeon@gnu.ac.kr
Format Journal Article
LanguageKorean
Published 01.10.2007
Subjects
BAT2
C4B
cSNP
Direct sequencing
GENOTIPOS
GENOTYPE
GENOTYPES
Genotyping
Multiplex-ARMS
SLA
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Summary:C4B and BAT2, assigned to the SLA class Ⅲ region, were recently reported on relation with human diseases. The primers for RT-PCR and RACE-PCR for CDS analysis of these genes of pig were designed by aligning the CDSs of humans and mice from GenBank. After we amplified and sequenced with these primers and cDNAs, the full-length CDSs of pig were determined. The CDS lengths of C4B and BAT2 were shown as 5226 bp and 6501 bp. In addition, the identities of nucleotide sequences with human and mouse were 76% to 87%, and the identities of amino acids were 72% to 90%.
Bibliography:2007003911
L10
ISSN:1598-9429