Detection method of Genetic Variation of Mulberry Dwarf Phytoplasma by PCR-SSCP Analysis
Single-strand conformation polymorphism (SSCP) analysis of MD and JWB phytoplasma isolates which amplified PCR products using the R16F2n/R2 phytoplasma universal primer pair were compared for variations of their nucleotide sequence. The MD and JWB phytoplasmas were clearly distinct each of the band...
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Published in | Journal of Korean Forestry Society Vol. 95; no. 6 |
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Main Authors | , , |
Format | Journal Article |
Language | Korean |
Published |
01.12.2006
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Subjects | |
Online Access | Get more information |
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Summary: | Single-strand conformation polymorphism (SSCP) analysis of MD and JWB phytoplasma isolates which amplified PCR products using the R16F2n/R2 phytoplasma universal primer pair were compared for variations of their nucleotide sequence. The MD and JWB phytoplasmas were clearly distinct each of the band patterns from about 1.2 kb PCR products. To clearly distinct of close SSCP band patterns, the MD and JWB phytoplasma PCR products were mixed and performed to detect their polymorphism. |
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Bibliography: | 2007001016 K01 |
ISSN: | 0445-4650 |