Characterization of Expressed Sequence Tags (ESTs) Generated from the Bombyx mandarina Whole Larvae and Molecular Cloning of Serine Protease Homologue Gene

We constructed an oligo-d(T) primed directional cDNA library from the Bombyx mandarina whole larvae. In an effort to isolate genes expressed in the B. mandarina, 227 expressed sequence tags (ESTs) were generated by single-pass sequencing from the cDNA library. Sequence analysis showed that 107 clone...

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Published inInternational Journal of Industrial Entomology Vol. 9; no. 2
Main Authors Hwang, J.S.;Yun, E.Y.;Goo, T.W.;Kim, I.S.;Choi, K.H.;Kim, K.Y.;Kang, S.W.(National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea)E-mail:hwangjs@rda.go.kr, Seong, S.I.(The University of Suwon, Hwaseong, Republic of Korea), Lee, S.M.(Miryang National University, Miryang, Republic of Korea)
Format Journal Article
LanguageEnglish
Subjects
Bombyx mandarina
ESTs
Midgut
PROTEASAS
PROTEASE
PROTEASES
SERINA
SERINE
Serine protease
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Summary:We constructed an oligo-d(T) primed directional cDNA library from the Bombyx mandarina whole larvae. In an effort to isolate genes expressed in the B. mandarina, 227 expressed sequence tags (ESTs) were generated by single-pass sequencing from the cDNA library. Sequence analysis showed that 107 clones (47.1%) were classified into known genes and 120 clones (52.9%) were novel transcripts, which are unknown for their function. Of the 107 known genes, the most abundant gene was found to be actin and followed by serine protease in the expression profile. Among these clones, a serine protease homolog (BmSP) which is a class of proteolytic enzymes isolated.
Bibliography:2005010546
L01
ISSN:1598-3579