Expression of a Recombinant Cry1Ac Crystal Protein Fused with a Green Fluorescent Protein in Bacillus thuringiensis subsp. kurstaki Cry-B

To investigate the co-expression and crystallization of a fusion gene between the Bacillus thuringiensis crystal protein and a foreign protein in B. thuringiensis, the expression of the Cry1Ac fused with green fluorescent protein (GFP) genes in a B. thuringiensis Cry-B strain was examined. The cry1A...

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Published inThe journal of microbiology Vol. 42; no. 4
Main Author Roh, J.Y.;Lee, I.H.;Li, M.S.;Chang, J.H.;Choi, J.Y.;Boo, K.S.;Je, Y.H.(Seoul National University, Seoul, Republic of Korea)E-mail:btrus@snu.ac.kr
Format Journal Article
LanguageEnglish
Subjects
Bacillus thuringiensis
Cry1Ac
fusion protein
GFP
recombinant
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Summary:To investigate the co-expression and crystallization of a fusion gene between the Bacillus thuringiensis crystal protein and a foreign protein in B. thuringiensis, the expression of the Cry1Ac fused with green fluorescent protein (GFP) genes in a B. thuringiensis Cry-B strain was examined. The cry1Ac gene was cloned in the B. thuringiensis-E. coli shuttle vector, pHT3101, under the control of the native cry1Ac gene promoter, while the GFP gene was inserted into the XhoI site upstream of the proteolytic cleavage site, in the middle region of the cry1Ac gene (pProAc-GFP). The B. thuringiensis Cry-B strain carrying pProAc-GFP (ProAc-GFP/CB) did not produce any inclusion bodies.
Bibliography:2005010452
L50
ISSN:1225-8873
1976-3794