Optimization of Culture Condition of Gluconacetobacter hansenii TF-2 for Cellulose Gel Production

• Published in: Han'guk Sikp'um Yŏngyang Kwahakhoe chi Vol. 33; no. 1
• Main Authors: Choi, K.H.;Jeong, J.S.(Catholic University of Daegu, Gyungsan, Republic of Korea)E-mail:khchoi@cu.ac.kr, Moon, C.H.;Kim, M.L.(Kyongbuk College of Science, Gyeongbuk, Republic of Korea)
• Format: Journal Article
• Language: Korean
• Subjects: bacterial cellulose; CELLULOSE; CELULOSA; CITRUS; citrus gel production; Gluconacetobacter hansenii TF-2; PRODUCCION; PRODUCTION; tea fungus; 감귤과즙; 겔 생산; 셀루로스
• ISSN: 1226-3311

Gluconacetobacter hansenii TF-2, an isolate from black tea fungus, was statically cultivated to ferment cellulose gel from citrus juice. The juice prepared by press filtering of peeled citrus fruit contained 135.5 ㎎ of total sugar/mL, 1.23% of total acid, and average pH of the juice was 3.98. The bacterium produced cellulose gel optimally on the surface of culture broth containing 17% of citrus juice and 10°Brix of total sugar. The optimum temperature was 30℃ for producing acetic acid and gel formation. The bacterium could not produce acetic acid on gel formation at 40℃. The optimum pH was 3.0∼4.0 but was not significantly different between pH 3.0∼4.0. The cultivation for 18 days under optimal conditions produced gel as 14.2±0.6 ㎜ of thickness and acids equivalent to 1.90±0.22% of acetic acid.