Nuclear Remodeling and In Vitro Development Following Somatic Cell Nuclear Transfer in Swine

This study was conducted to investigate nuclear remodeling and developmental rate following nuclear transfer of fetal fibroblast cells, ear skin cells and oviduct epithelial cells into porcine recipient oocytes. To test parthenogenetic activation, oocytes were treated with a 6-dimethylaminopurine (6...

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Published inReproductive & developmental biology Vol. 28; no. 4
Main Authors Yoon, J.T.;Min, K.S.;Hwang, S.S.(Hankyong National University, Ansung, Republic of Korea)E-mail:jtyoon@hnu.hankyong.ac.kr, Kim, Y.Y.;Lee, J.W.(Kongju National University, Kongju, Republic of Korea)
Format Journal Article
LanguageEnglish
Subjects
ANIMAL EMBRYOS
Blastocyst
CELLS
CELLULE
CELULAS
EMBRIONES ANIMALES
EMBRYON ANIMAL
ESTIMULO
Nuclear remodeling
Somatic cell
Stimulation
STIMULI
STIMULUS
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Summary:This study was conducted to investigate nuclear remodeling and developmental rate following nuclear transfer of fetal fibroblast cells, ear skin cells and oviduct epithelial cells into porcine recipient oocytes. To test parthenogenetic activation, oocytes were treated with a 6-dimethylaminopurine (6-DMAP), a single DC-pulse (DC), calcium ionomycin (ionomycin), DC+6-DMAP and ionomycin + 6-DMAP after in vitro maturation. For nuclear transfer, in vitro matured oocytes were enucleated, and donor cells were transferred into oocytes. Cloned embryos were fused and stimulated with 6-MDAP for 4 h and cultured in vitro for 6 days.
Bibliography:2005008869
L53
ISSN:1738-2432