Retrovirus Vector-Mediated Construction of Porcine Cell Lines Expressing Human PTH

Human PTH (parathyroid hormone) is known to be efficacious for curing osteoporesis. In this study, we attempted to construct genetically modified porcine cell lines that can ultimately be used for donor cells in nuclear transfer-mediated transgenesis. By using retrovirus vectors carrying tetracyclin...

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Published inReproductive & developmental biology Vol. 28; no. 3
Main Authors Jeong, J.Y.;Koo, B.C.;Kim, N.H.(Chungbuk National University, Cheongju, Republic of Korea), Kwon, M.S.;Park, S.H.;Kim, T.O.(Catholic University of Daegu, Gyeongsan, Republic of Korea)E-mail:takim@cu.ac.kr
Format Journal Article
LanguageKorean
Subjects
CONSTRUCCION
CONSTRUCTION
hPTH
Retrovirus vector
Retrovirus Vector System
Tet-On system
VECTEUR DE MALADIE
VECTORES
VECTORS
WPRE
구축
돼지 세포
발현
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Summary:Human PTH (parathyroid hormone) is known to be efficacious for curing osteoporesis. In this study, we attempted to construct genetically modified porcine cell lines that can ultimately be used for donor cells in nuclear transfer-mediated transgenesis. By using retrovirus vectors carrying tetracycline-regulatory expression system and WPRE (woodchuck hepatitis virus posttranscriptional regulatory element) sequence, we could control PTH expression with tetracycline and boost the promoter activity. Considering that low or constitutive expression of the transgene has been one of major problems that needs to be solved in transgenic animal studies, our results could be helpful in successful production of transgenic pigs as bioreactors.
Bibliography:2005008863
L53
ISSN:1738-2432