Identification of Genes for Growth Oxygen in Escherichia coli by Operon Fusion and Southern Blot Techniques

Seven Escherichia coli cells defective with aerobic growth were isolated by the insertion of ¥ëplaceMu53, a hybrid bacteriophage of ¥ë and Mu, which created a transcriptional fusion to lacZY. These insertion mutant cells were tested on an XG medium for anaerobic expression of lacZ by fusion to a pro...

Full description

Saved in:
Bibliographic Details
Published inJournal of Microbiology and Biotechnology Vol. 13; no. 6
Main Authors Kim, I.M, Lee, T.C, Won, J.S, Choe, M.Y. (Korea University, Seoul, Republic of Korea)
Format Journal Article
LanguageEnglish
Published 01.12.2003
Subjects
escherichia coli
Online AccessGet more information

Cover

More Information
Summary:Seven Escherichia coli cells defective with aerobic growth were isolated by the insertion of ¥ëplaceMu53, a hybrid bacteriophage of ¥ë and Mu, which created a transcriptional fusion to lacZY. These insertion mutant cells were tested on an XG medium for anaerobic expression of lacZ by fusion to a promoter. The chromosomal DNA from these strains were digested by EcoRI, and the EcoRI fragments that contained were digested by EcoRI, and the EcoRI fragments that contained the fused gene and lacZ sequence were identified by Southern hybridization , using lacZ sequence were identified by Southern hybridization , using lacZ containing plasmid as a probe
Bibliography:2004005418
L01
ISSN:1078-7825