RFLP patterns detected by the transposons, mag and K 1.4, in silkworm [Bombyx mori] races

We first examined whether an appropriate RFLP pattern for studying the genetic relationships among silkworm races can be obtained by suing DNA sequences of the transposons, mag and k1.4, as probes. Comparison of the RFLP patterns among the representative geographical races showed that highly polymor...

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Bibliographic Details
Published inGamma field symposia no. 32
Main Authors Tamura, T. (National Inst. of Sericultural and Entomological Science, Tsukuba, Ibaraki (Japan)), Kanda, T, Yukuhiro, K, Yasukochi, Y, Hinomoto, N, Shimizu, K, Kosegawa, E, Okajima, T
Format Journal Article
LanguageEnglish
Published 01.12.1994
Subjects
BOMBYX MORI
FRAGMENTACION DEL ADN
FRAGMENTATION DE L'ADN
RACE (ANIMAL)
RAZAS (ANIMALES)
RFLP
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
TRANSPOSICION DE GENES
TRANSPOSITION DE GENES
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Summary:We first examined whether an appropriate RFLP pattern for studying the genetic relationships among silkworm races can be obtained by suing DNA sequences of the transposons, mag and k1.4, as probes. Comparison of the RFLP patterns among the representative geographical races showed that highly polymorphic bands appeared in these sequences when digested by restriction enzymes which recognized both ends of the transposons. A total of 25 bands were observed in the patterns, of which 18 were polymorphic. Genetic similarly and association among the races used were calculated from the patterns. The values obtained agreed very well with the relationships from historical descriptions
Bibliography:F30
9602386
ISSN:0435-1096