Identification of environmental filamentous fungi based on carbon source utilization and improvement of identification accuracy

To identify filamentous fungi in the indoor air, MicroLog system (BIOLOG Inc.), which is based on the utilization of carbon sources, was used. MicroLog system is equipped with a plastic plate containing 96 wells, 95 of which were filled with 95 different carbon sources. The plate was inoculated with...

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Bibliographic Details
Published inJournal of Antibacterial and Antifungal Agents (Japan) Vol. 38; no. 6
Main Authors Konuma, R., Tokyo-to. Industrial Technology Research Inst. (Japan), Watanabe, M, Hara Kudo, Y, Sugita Konishi, Y, Takatori, K, Ichinoe, M, Kawarada, K
Format Journal Article
LanguageJapanese
Published 01.06.2010
Subjects
APPROPRIATE TECHNOLOGY
CARBON
CARBONE
CARBONO
CHAMPIGNON
FUNGI
HONGOS
http://www.fao.org/aos/agrovoc#c_12522
http://www.fao.org/aos/agrovoc#c_1301
http://www.fao.org/aos/agrovoc#c_3145
http://www.fao.org/aos/agrovoc#c_3791
http://www.fao.org/aos/agrovoc#c_544
http://www.fao.org/aos/agrovoc#c_6077
IDENTIFICACION
IDENTIFICATION
METHODOLOGIE
METHODOLOGY
METODOLOGIA
POLLUTION
POLUCION
TECHNOLOGIE APPROPRIEE
TECNOLOGIA APROPIADA
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Summary:To identify filamentous fungi in the indoor air, MicroLog system (BIOLOG Inc.), which is based on the utilization of carbon sources, was used. MicroLog system is equipped with a plastic plate containing 96 wells, 95 of which were filled with 95 different carbon sources. The plate was inoculated with the conidial suspension of an isolate. After incubation, the plate was evaluated with a microplate reader in order to obtain the profiles of growth responses, i.e., a metabolic fingerprint for each isolate. For species identification, the profiles were then compared with those in the Biolog database. In our study, the identification accuracy was determined by using 30 isolates of 30 species belonging to 4 genera of environmental filamentous fungi, namely, Aspergillus, Eurotium, Penicillium, and Cladosporium. The results showed that for the genus Aspergillus, 9 of the 11 (81.8%) species were identified correctly up to the species level. The percentage of identification accuracy for the genera Penicillium and Cladosporium were 41.7% and 100%, respectively. Thus, the calibration of the Biolog database, which contains isolates whose origins have been identified, was found to increase the accuracy of the identification of environmental filamentous fungi that were examined in this study.
Bibliography:F40
2010005346
ISSN:0385-5201