Improvement of vaccination effect on ocellate puffer and Japanese flounder by the feeding of artificial feed with heat-killed Enterococcus faecalis FK-23

• Published in: Suisan Zoshoku Vol. 56; no. 3
• Main Authors: Kotani, T.(Fukuyama Univ., Onomichi, Hiroshima (Japan)), Kitamoto, E, Kurata, O, Hirayama, N, Fushimi, H, Satoh, S, Hatai, K, Miyashima, A, Nohmi, T, Shimada, T
• Format: Journal Article
• Language: Japanese
• Published: 01.09.2008
• Subjects: ALIMENT COMPOSE POUR ANIMAUX; ALIMENTACION DE PECES; ALIMENTATION DES POISSONS; COMPOUND FEEDS; ENTEROCOCCUS; FISH FEEDING; HEAT TREATMENT; PARALICHTHYS OLIVACEUS; PIENSOS COMPUESTOS; TETRAODONTIDAE; TRAITEMENT THERMIQUE; TRATAMIENTO TERMICO; VACCINATION; VACUNACION
• ISSN: 0371-4217

We studied the specific immunostimulant effect of heat-killed Enterococcus faecalis FK-23 (FK-23) when the artificial diet including FK-23 was fed on Japanese flounder and ocellate puffer. The diets with 5 different concentrations of FK-23 was prepared (0-1.25%). Fishes were injected formalin-killed cells of Vibrio anguillarum and the injection was performed two times. The second injection was done 3 weeks after the first injection. The agglutination titer of antibody for V. anguillarum cells in their serum was evaluated every week after the first injection and the evaluation was performed six times. The agglutination titer in the serum of ocellate puffer fed the diet including highest concentration (1.25%) of FK-23 increased more intensively with the progress of rearing than other concentrations. However those puffers fed the diet with 1.25% of FK-23 did not grow during the experimental rearing period. Although the agglutination titer in the serum of flounder did not increase, this is possibly because the non-specific immunity worked more intensively than specific one. Consequently FK-23 is possible to increase the vaccination effect of puffer and maintain its effect, and it also makes the non-specific immunity of flounder intensify.