Some proposals for developing the more precise and critical studies on newer vitamin B6 physiology

SUMMARY In order to develop more precise and critical studies on newer vitamin B sub6 physiology, some proposals, must be resolved in near future, are reviewed. First of all, analytical task should be completed is the establishment of more simple and concise HPLC method being able to analyze simulta...

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Bibliographic Details
Published inResearch Bulletin of the Faculty of Agriculture - Gifu University (Japan) no. 68
Main Author Tsuge, H. (Gifu Univ. (Japan). Faculty of Agriculture)
Format Journal Article
LanguageJapanese
Published 01.12.2003
Subjects
ANALYTICAL METHODS
PHYSIOLOGICAL FUNCTIONS
PYRIDOXINE
VITAMINS B
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Summary:SUMMARY In order to develop more precise and critical studies on newer vitamin B sub6 physiology, some proposals, must be resolved in near future, are reviewed. First of all, analytical task should be completed is the establishment of more simple and concise HPLC method being able to analyze simultaneously at least 7 derivatives of vitamin B sub6 (pyridoxal, pyridoxamine, pyridoxine, those of 5'-phosphate ester, and pyridoxic acid), which are ubiquitously found in the biological materials. Newer method to analyze these vitamin B sub6 derivatives using one isocratic HPLC column with high sensitivity is necessitated. Thus, stable and synthetic compound suitable as an inner standard, which possesses similar characteristics with these natural compounds but not easily decomposed during the sample preparation, is needed for the development of accurate and reliable HPLC analysis. Another problems should be solved are the establishment of modulation mechanisms between vitamin B sub6 , especially PLP, and transcription factors during gene expression under physiological conditions.
Bibliography:U30
2004002720
ISSN:0072-4513