"Efficient transformation of rice [Oryza sativa] cultivar ""Koshihikari"" mediated by Agrobacterium tumefaciens."

"Recently, the methods for transformation of rice plants mediated by Agrobacterium tumefaciens have frequently been employed. However, most studies on transformation of rice by binary-vector derived from pBI101 were carried out for the limited cultivars, which were easy to culture. We attempted...

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Bibliographic Details
Published inBulletin of the Mie Agricultural Technical Center (Japan) no. 27
Main Authors "Hashidume, F. (Mie-ken. Agricultural Technical Center, Ureshino (Japan)), Tsuchiya, T, Ugaki, M, Kowyama, Y.
Format Journal Article
LanguageJapanese
Published 01.03.2000
Subjects
AGROBACTERIUM TUMEFACIENS
CALLUS
CULTURE MEDIA
EFFICIENCY
EMBRYONIC DEVELOPMENT
GENETIC TRANSFORMATION
ORYZA SATIVA
VARIETIES
VECTORS
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Summary:"Recently, the methods for transformation of rice plants mediated by Agrobacterium tumefaciens have frequently been employed. However, most studies on transformation of rice by binary-vector derived from pBI101 were carried out for the limited cultivars, which were easy to culture. We attempted the cultural conditions to transform a major cv, ""Koshihikari"" by pIG121Hm derived from pBI101 and found that the inducing period of scutella-calli was of importance. Thus the efficient production of transformants could be attained when the scutella-calli were induced for about three weeks on improved callus-inducing media and co-cultured with Agrobacterium tumefaciens."
Bibliography:F30
2003000378
ISSN:0388-8290