Spatial expression and characterization of a putative ethylene receptor protein NTHK1 in tabacco [Nicotiana tabacum]

A putative ethylene receptor gene NTHK1 encodes a protein with a putative signal peptide, three transmembrane segments, a putative histidine kinase domain and a putative receiver domain. The receiver domain was expressed in an Escherichia coli expression system, purified and used to generate polyclo...

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Published inPlant and cell physiology Vol. 43; no. 7
Main Authors Xie, C. (Chinese Academy of Sciences, Beijing (China)), Zhang, Z.G, Zhang, J.S, He, X.J, Cao, W.H, He, S.J, Chen, S.Y
Format Journal Article
LanguageEnglish
Published 01.07.2002
Subjects
ESCHERICHIA COLI
ETHYLENE
GENE EXPRESSION
HORMONE RECEPTORS
IMMUNOLOGICAL TECHNIQUES
NICOTIANA TABACUM
POLYCLONAL ANTIBODIES
PROTEINS
TRANSGENIC PLANTS
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Summary:A putative ethylene receptor gene NTHK1 encodes a protein with a putative signal peptide, three transmembrane segments, a putative histidine kinase domain and a putative receiver domain. The receiver domain was expressed in an Escherichia coli expression system, purified and used to generate polyclonal antibodies for immunohistochemistry analysis. The spatial expression of the NTHK1 protein was then investigated. We found that NTHK1 was abundant during flower and ovule development. It was also expressed in glandular hairs, stem, and in leaves that had been wounded. The NTHK1 gene was further introduced into the tobacco plant and we found that, in different transgenic lines, the NTHK1 gene was transcribed to various degrees. Upon ACC treatment, the etiolated transgenic seedlings showed reduced ethylene sensitivity when compared with the control, indicating that NTHK1 is a functional ethylene receptor in plants.
Bibliography:F60
2002006506
ISSN:0032-0781
1471-9053