Characterization of tomato lines by RAPD-PCR and flow cytometry [Random Amplified Polymorphic DNA - polymerase Chain Reaction]

Fifteen hybrid F1 tomato lines and their parental lines have been characterized by RAPD-PCR and flow cytometry. The RAPD-PCR analysis was based on amplification of markers generated by random arbitrary 21-mer primers. This technique detected individual differences among all but two lines included in...

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Published inAnnali della Facolta' di Scienze Agrarie della Universita' degli Studi di Napoli Federico II - Portici (Italy) Vol. 29
Main Authors Boiano, S, Cottone, C, Viscardi, M, D'Apice, L, Rosa, G. de, Capparelli, R, Iannelli, D. (Naples Univ., Portici (Italy)), Spigno, P. (Cirio Ricerche, Piana di Monteverna, Caserta (Italy)), Noviello, C. (Naples Univ., Portici (Italy). Istituto di Patologia Vegetale)
Format Journal Article
LanguageItalian
Published 1995
Subjects
ADN
AMELIORATION DES PLANTES
CITOGENETICA
CYTOGENETIQUE
DISTANCE GENETIQUE
DISTANCIA GENETICA
FITOMEJORAMIENTO
FLUORIMETRIA
FLUORIMETRIE
GENIE GENETIQUE
HIBRIDOS
HYBRIDE
IDENTIFICACION
IDENTIFICATION
INGENIERIA GENETICA
LYCOPERSICON ESCULENTUM
MARCADORES GENETICOS
MARQUEUR GENETIQUE
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Summary:Fifteen hybrid F1 tomato lines and their parental lines have been characterized by RAPD-PCR and flow cytometry. The RAPD-PCR analysis was based on amplification of markers generated by random arbitrary 21-mer primers. This technique detected individual differences among all but two lines included in the study. Flow cytometry detected individual differences in the DNA content of the majority of lines. The two techniques of RAPD-PCR and flow cytometry complement each other, and appear particularly informative in the analysis of plant genome Mediante RAPD-PCR e citofluorimetria a flusso sono state caratterizzate 15 linee ibride di pomodoro e le rispettive linee parentali. L'analisi RAPD-PCR, basata sull'amplificazione di marcatori genetici con sei primers di 21 nucleotidi con sequenza arbitraria, ha consentito di mettere in evidenza caratteristiche differenze genetiche in 28/30 linee di pomodoro studiate. L'analisi citofluorimetrica ha permesso di caratterizzare le linee anche in base al contenuto in DNA. Il lavoro ha messo in luce la capacita' della tecnica della RAPD-PCR e della citofluorimetria di complementarsi e risultare particolarmente utili nel miglioramento genetico
Bibliography:F30
9760424
U30
ISSN:0365-799X