In vitro nematicidal activity of extracts of bulbs and seeds of onion against root-knot nematode Meloidogyne incognita

Among the various solvent extracts of the bulbs and seeds of onion assayed for in vitro nematicidal activity against root-knot nematode, Meloidogyne incognita, hot methanol extract of defatted seeds exhibited significant activity. The active extract contained a compound which fluoresced in ultra vio...

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Published inNematologia mediterranea Vol. 22; no. 1
Main Authors Nidiry, E.S.J, Chandravadana, M.V, Rao, M.S. (Indian Inst. of Horticultural Research, Bangalore), Khan, R.M. (Central Horticultural Experimental Station, Ranchi (India))
Format Journal Article
LanguageEnglish
Published 01.06.1994
Subjects
ACIDE ASPARTIQUE
ACIDO ASPARTICO
BULBE
BULBO
CEBOLLA
EXPERIMENTACION IN VITRO
EXPERIMENTATION IN VITRO
EXTRACTOS
EXTRAIT
LEUCINA
LEUCINE
MELOIDOGYNE INCOGNITA
METANOL
METHANOL
METHIONINE
METIONINA
OIGNON
RACINE
RAICES
SEMENCE
SEMILLAS
THREONINE
TREONINA
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Summary:Among the various solvent extracts of the bulbs and seeds of onion assayed for in vitro nematicidal activity against root-knot nematode, Meloidogyne incognita, hot methanol extract of defatted seeds exhibited significant activity. The active extract contained a compound which fluoresced in ultra violet light; it coloured pink when sprayed with diazotized sulphanilic acid followed by sodium hydroxide on a TLC plate. Free amino acids viz. leucine (isoleucine), aspartic acid, methionine and threonine each exhibited nematicidal activity, but to a much lesser extent than the extract itself [Tra i diversi estratti mediante solventi dei bulbi e dei semi di cipolla testati per l'attivita' nematocida in vitro contro il nematode galligeno Meloidogyne incognita, il metanolo caldo estratto da semi essiccati ha mostrato un'attivita' significativa. L'estratto attivo conteneva un composto che fluoresceva in luce ultra-violetta. Si colorava di rosa se spruzzato con acido sulfanilico diazotato seguito da idrossido di sodio su una superficie TLC. Gli amminoacidi liberi come la leucina (isoleucina), l'acido aspartico, la metionina e la treonina hanno mostrato, ciascuno, attivita' nematocida, ma in misura molto inferiore all'estratto stesso]
Bibliography:9462523
H10
ISSN:0391-9749